Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23764-1] to Complement factor 8 beta/C8B - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free
    See all Complement factor 8 beta/C8B primary antibodies

  • Description

    Rabbit monoclonal [EPR23764-1] to Complement factor 8 beta/C8B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney, liver and large intestine tissue lysates; Human, mouse and rat serum; LIF-tagged human C8B recombinant protein. IHC-P: Human thyroid and liver tissue. IP: Human kidney tissue lysate.

  • General notes

    ab278049 is the carrier-free version of ab278045. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278049 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    All lanes : Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (ab278045) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Human large intestine tissue lysate
    Lane 4 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:21750743).

    Negative: human brain (PMID:16483657, PMID:27005612).

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 µg/ml) dilution followed by a ready to use secondary antibody. Positive staining on the blood plasma in human thyroid (PMID: 9476128). The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary antibody.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Immunoprecipitation - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Complement factor 8 beta/C8B was immunoprecipitated from 0.35 mg human kidney tissue lysate with ab278045 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab278045 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney tissue lysate 10 µg

    Lane 2: ab278045 IP in human kidney tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278045 in human kidney tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (ab278045) at 1/1000 dilution + Human serum at 20 µl

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:21750743).

    Exposure time: 15 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 µg/ml) dilution  followed by a ready to use secondary antibody. Cytoplasmic staining on human liver. The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary antibody).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    All lanes : Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (ab278045) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Rat serum

    Lysates/proteins at 20 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:21750743).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 26 seconds.

  • Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Western blot - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    All lanes : Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] (ab278045) at 1/1000 dilution

    Lane 1 : LIF-tagged human C8B recombinant protein, 30 ng
    Lane 2 : E.coli extracts containi his-tagged human C8A recombinant protein, 30 ng
    Lane 3 : E.coli extracts containi his-tagged human C9 recombinant protein, 30 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa



    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody has no cross reaction with human C8A and C9.

    Sample loaded onto lane 1 was purified protein extracted from HEK293 expressing C8B, which contains a LIF-tag (40 kDa). 

    C8A and C9 recombinant proteins were expressed in E.coil expression systems.

    Exposure time: 26 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

    This data was developed using ab278045, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Complement factor 8 beta/C8B with ab278045 at 1/1000 (0.533 µg/ml) dilution followed by a ready to use secondary antibody. The section was incubated with ab278045 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: no staining on human cerebrum.

    Secondary antibody only control: Secondary antibody is a ready to use secondary antibody.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)
    Anti-Complement factor 8 beta/C8B antibody [EPR23764-1] - BSA and Azide free (ab278049)

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