Anti-CLEC4C antibody [EPR21985] (ab239078)
![Anti-CLEC4C antibody [EPR21985] (ab239078)](https://www.abcam.com/ps/products/239/ab239078/Images/ab239078-319058-239078IHC1a.jpg "Anti-CLEC4C antibody [EPR21985] (ab239078)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21985] to CLEC4C
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CLEC4C antibody [EPR21985]
See all CLEC4C primary antibodies -
Description
Rabbit monoclonal [EPR21985] to CLEC4C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CLEC4C aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q8WTT0 -
Positive control
- IHC-P: Human tonsil tissue; Mouse and rat spleen tissues. Flow Cyt: Primary human peripheral blood mononuclear cell (PBMC).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21985 -
Isotype
IgG
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of human tonsil (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (ab239078)](https://www.abcam.com/ps/products/239/ab239078/Images/ab239078-319062-239078FC1.jpg)
Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (ab239078)Flow cytometric analysis of primary human peripheral blood mononuclear cell (PBMC) labeling CLEC4C with ab239078 at 1/60 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG Fc (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Note: CLEC4C and CD303 are alternative names for the same target.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)](https://www.abcam.com/ps/products/239/ab239078/Images/ab239078-319060-239078IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of mouse spleen (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)](https://www.abcam.com/ps/products/239/ab239078/Images/ab239078-319061-239078IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of rat spleen (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)](https://www.abcam.com/ps/products/239/ab239078/Images/ab239078-319059-239078IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: Negative staining in human cardiac muscle (PMID:11748283).
Counter stained with hematoxylin.
