Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
![Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)](https://www.abcam.com/ps/products/215/ab215203/Images/ab215203-1-anti-cleaved-n-terminal-gsdmd-antibody-epr20829-408-western-blot-a431-human.jpg "Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20829-408] to cleaved N-terminal GSDMD
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] -
Description
Rabbit monoclonal [EPR20829-408] to cleaved N-terminal GSDMD -
Host species
Rabbit -
Specificity
The N-term cleaved Gasdermin-D needs inflammatory caspases in response to canonical and non-canonical inflammasome activators (PubMed: 31548300, PubMed: 27418190, PubMed: 26375259). If need to detect cleavage of GSDMD, please ensure that the samples are treated with inflammation before testing.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Salmonella Infected (MOI:100) A431 whole cell lysate; THP-1 treated with 500 ng/ml EprI for 2h, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20829-408 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203) at 1/1000 dilution + Salmonella Infected (MOI:100) A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesLysate was kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with literature (PMID: 26375003).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)](https://www.abcam.com/ps/products/215/ab215203/Images/ab215203-2-anti-cleaved-n-terminal-gsdmd-antibody-epr20829-408-western-blot-thp1-human.jpg)
Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)All lanes : Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : THP-1 treated with 500 ng/ml EprI for 2h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesLysate was kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with literature (PMID: 26375003).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)](https://www.abcam.com/ps/products/215/ab215203/Images/ab215203-3-benefits-of-recombinant-antibodies.png)
Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
