Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20829-408] to cleaved N-terminal GSDMD
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

  • Description

    Rabbit monoclonal [EPR20829-408] to cleaved N-terminal GSDMD

  • Host species

    Rabbit

  • Specificity

    The N-term cleaved Gasdermin-D needs inflammatory caspases in response to canonical and non-canonical inflammasome activators (PubMed: 31548300, PubMed: 27418190, PubMed: 26375259). If need to detect cleavage of GSDMD, please ensure that the samples are treated with inflammation before testing.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Salmonella Infected (MOI:100) A431 whole cell lysate; THP-1 treated with 500 ng/ml EprI for 2h, whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20829-408

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
    Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
    Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203) at 1/1000 dilution + Salmonella Infected (MOI:100) A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Lysate was kindly provided by Dr Feng Shao.

    The molecular weight observed is consistent with literature (PMID: 26375003).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
    Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
    All lanes : Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : THP-1 treated with 500 ng/ml EprI for 2h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 31 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Lysate was kindly provided by Dr Feng Shao.

    The molecular weight observed is consistent with literature (PMID: 26375003).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)
    Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (ab215203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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