Anti-Citrulline antibody (ab100932)
Key features and details
- Rabbit polyclonal to Citrulline
- Suitable for: ELISA, ICC
- Reacts with: Species independent
- Isotype: IgG
Overview
-
Product name
Anti-Citrulline antibody
See all Citrulline primary antibodies -
Description
Rabbit polyclonal to Citrulline -
Host species
Rabbit -
Specificity
ab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites. -
Tested applications
Suitable for: ELISA, ICCmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Species independent -
Immunogen
Citrulline coupled to KLH via Glutaraldehyde
-
Positive control
- Hypercitrullinated Histones induced in HL60 cells by DMSO +calcium +calcium ionophore A23187
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ELISA - Anti-Citrulline antibody (ab100932)
ELISA data showing the high immuno-reactivity of ab100932 against citrulline due to the high amounts of citrulline conjugated to BSA.
-
ELISA - Anti-Citrulline antibody (ab100932)ELISA data demonstrating the specificity of ab100932 to citrulline naturally present in histones (endogenous citrulline).
-
Immunocytochemistry - Anti-Citrulline antibody (ab100932) This image is courtesy of an anonymous AbreviewImmunocytochemical analysis of Human buccal mucosal cells, staining Citrulline with ab100932.
Cells were fixed with acetone followed by 2% glutaraldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 15 hours at 4°C. An undiluted biotinylated anti-rabbit polyclonal IgG was used as the secondary antibody.
