Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68)
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Chk2 (phospho T68) antibody [Chk2T68-D12]
See all Chk2 primary antibodies
Description

Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Chk2 (phospho T68). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O96017

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Positive control
- WB: HEK-293 (treated with UV) cell extract. Flow cyt: NIH/3T3 cells; HEK-293T and HeLa cells (both treated with UV/TPA); C6 cells (treated with pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

Chk2T68-D12
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

ab278548 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278548 (green).
Western blot - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

All lanes : Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548) at 1 µg/ml

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) untreated, whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) treated with UV, whole cell lysate

Predicted band size: 61 kDa
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

Flow cytometric analysis of Hela cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using Pab278548, at 0.01 ug/mL, CHK2T68-D12 #2116, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with lambda phosphatase (black) or treated with UV+TPA (blue).
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or untreated (red) or UV/TPA-treated (green) or untreated and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using ab278548 at 0.1µg/mL.
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 µg/mL ab278548.
Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)

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