Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)
![Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-4-chk2t68d123t3.jpg "Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68)
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Chk2 (phospho T68) antibody [Chk2T68-D12]
See all Chk2 primary antibodies -
Description
Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Chk2 (phospho T68). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O96017 -
Positive control
- WB: HEK-293 (treated with UV) cell extract. Flow cyt: NIH/3T3 cells; HEK-293T and HeLa cells (both treated with UV/TPA); C6 cells (treated with pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Chk2T68-D12 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)ab278548 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278548 (green).
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![Western blot - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-455167-anti-chk2-phospho-t68-antibody-chk2t68-d12-western-blot-uv-treated-hek293-human.jpg)
Western blot - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)All lanes : Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) untreated, whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) treated with UV, whole cell lysate
Predicted band size: 61 kDa
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![Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-455807-AntiChk2-phospho-T68-antibody-Chk2T68D12-flow-cytometry.JPG)
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)Flow cytometric analysis of Hela cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using Pab278548, at 0.01 ug/mL, CHK2T68-D12 #2116, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells treated with lambda phosphatase (black) or treated with UV+TPA (blue).
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![Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-3-chk2t68d12pepblock.jpg)
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or untreated (red) or UV/TPA-treated (green) or untreated and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using ab278548 at 0.1µg/mL. -
![Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-5-ab278548comparisonflow.jpg)
Flow Cytometry - Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 µg/mL ab278548.
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![Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)](https://www.abcam.com/ps/products/278/ab278548/Images/ab278548-1-benefits-of-recombinant-antibodies.png)
Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548)