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Anti-Chk2 (phospho S516) antibody (ab36724)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Chk2 (phospho S516) antibody (ab36724)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Chk2 (phospho S516)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Chk2 (phospho S516) antibody
    See all Chk2 primary antibodies
  • Description

    Rabbit polyclonal to Chk2 (phospho S516)
  • Host species

    Rabbit
  • Specificity

    anti-CHK2(Phospho-Ser516) antibody detects endogenous levels of CHK2 only when phosphorylated at Serine 516.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of Serine 516 (Q-P-SP-T-S).

  • Positive control

    • UV treated Hela Cell extract.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Western blot - Anti-Chk2 (phospho S516) antibody (ab36724)
    Western blot - Anti-Chk2 (phospho S516) antibody (ab36724)
    All lanes : Anti-Chk2 (phospho S516) antibody (ab36724) at 1/500 dilution

    Lane 1 : Hela cell extract treated with UV
    Lane 2 : Hela cell extract untreated

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Lanes can be loaded with 5-30µg of total protein.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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