Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5528] to Chk2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Chk2 antibody [EPR5528] - BSA and Azide free
    See all Chk2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5528] to Chk2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248527 is the carrier-free version of ab133505 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)

    This data was developed using ab133505, the same antibody clone in a different buffer formulation.

    Lanes 1, 5, and 9: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 6, and 10: Chk2 knockout HAP1 cell lysate (20 µg)

    Lanes 3, 7, and 11: HeLa cell lysate (20 µg)

    Lanes 4, 8, and 12: HEK293 cell lysate (20 µg)

    Lanes 1, 2, 3, and 4: Green signal from target - ab133505 observed at 62 kDa

    Lanes 5, 6, 7, and 8: Red signal from loading control - ab8245 observed at 37 kDa

    Lanes 9, 10, 11, and 12: Merged (red and green) signal

    ab133505 was shown to specifically react with Chk2 when Chk2 knockout samples were used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab133505 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    All lanes : Anti-Chk2 antibody [EPR5528] (ab133505) at 1/10000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT29 lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    This data was developed using ab133505, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)

    This data was developed using ab133505, the same antibody clone in a different buffer formulation.

    Lanes 1: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2: Chk2 knockout HAP1 cell lysate (20 µg)

    Lanes 3: HeLa cell lysate (20 µg)

    Lanes 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133505 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab133505 and a competitor's rabbit polyclonal antibody.

  • OI-RD Scanning - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    OI-RD Scanning - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    This data was developed using ab133505, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)
    Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527)

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