Anti-PUMA antibody - C-terminal (ab227130)
Key features and details
- Rabbit polyclonal to PUMA - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PUMA antibody - C-terminal
See all PUMA primary antibodies -
Description
Rabbit polyclonal to PUMA - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PUMA (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9BXH1 -
Positive control
- WB: HCT 116 treated with cisplatin whole cell lysate; K562 and THP-1 whole cell extracts. IP: HCT 116 treated with cisplatin whole cell extract. IHC-P: Human hepatoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PUMA antibody - C-terminal (ab227130)All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) with mock treatment for 0 hours, whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) with 30 uM cisplatin treatment for 24 hours, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa15% SDS-PAGE gel.
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Western blot - Anti-PUMA antibody - C-terminal (ab227130)All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa12 % SDS-PAGE gel.
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Immunoprecipitation - Anti-PUMA antibody - C-terminal (ab227130)PUMA was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hours, whole cell extract with 4 µg of ab227130. Western blot was performed from the immunoprecipitate using ab227130. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: Preimmune Rabbit IgG instead of ab227130 in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.
Lane 2: ab227130 IP in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.
15 % SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody - C-terminal (ab227130)Paraffin-embedded human hepatoma tissue tissue stained for PUMA using ab227130 at 1/500 dilution in immunohistochemical analysis.
