Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)
![Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)](https://www.abcam.com/ps/products/271/ab271961/Images/ab271961-3-ab199031281276antichk2antibodyepr19236immunoprecipitation.jpg "Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19236] to Chk2 - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Chk2 antibody [EPR19236] - BSA and Azide free
See all Chk2 primary antibodies -
Description
Rabbit monoclonal [EPR19236] to Chk2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen and colon lysates; NIH/3T3, C2C12 and bEND.3 whole cell lysates. IHC: Mouse spleen and testis tissue.
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General notes
ab271961 is the carrier-free version of ab199031. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19236 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)
Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Exposure time 3 minutes.
Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).Lane 2: ab199031 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)](https://www.abcam.com/ps/products/271/ab271961/Images/ab271961-2-ab199031281274antichk2antibodyepr19236immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.
Nuclear staining on spermatogonia of mouse testis (PMID: 12192050, PMID: 11593395).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)](https://www.abcam.com/ps/products/271/ab271961/Images/ab271961-1-ab199031281268antichk2antibodyepr19236immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on mouse spleen is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.
Nuclear staining on mouse spleen (PMID: 12192050, PMID: 11593395).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199031). -
![Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)](https://www.abcam.com/ps/products/271/ab271961/Images/ab271961-4-benefits-of-recombinant-antibodies.png)
Anti-Chk2 antibody [EPR19236] - BSA and Azide free (ab271961)
