Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)
![Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)](https://www.abcam.com/ps/products/251/ab251477/Images/ab251477-385126-anti-chk2-antibody-epr19482-bsa-and-azide-free-western-blot-human-lysate.jpg "Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19482] to Chk2 - BSA and Azide free
- Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Chk2 antibody [EPR19482] - BSA and Azide free
See all Chk2 primary antibodies -
Description
Rabbit monoclonal [EPR19482] to Chk2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HeLa and MDA-MB-231 cell lysates.
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General notes
ab251477 is the carrier-free version of ab207446. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251477 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19482 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)All lanes : Anti-Chk2 antibody [EPR19482] (ab207446) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CHEK2 knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : MDA-MB-231 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab207446).
Lanes 1-4: Merged signal (red and green). Green - ab207446 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab207446 Anti-Chk2 antibody [EPR19482] was shown to specifically react with Chk2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264815 (knockout cell lysate ab257104) was used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab207446 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)](https://www.abcam.com/ps/products/251/ab251477/Images/ab251477-2-benefits-of-recombinant-antibodies.png)
Anti-Chk2 antibody [EPR19482] - BSA and Azide free (ab251477)
