Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17206(B)] to PRPF4
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PRPF4 antibody [EPR17206(B)]
    See all PRPF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17206(B)] to PRPF4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, SW480, Raji and Jurkat whole cell lysates; Human fetal spleen lysate. IHC-P: Human cervix carcinoma tissue. ICC/IF: HeLa and MCF7 cells. IP and Flow Cyt: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17206(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Western blot - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    All lanes : Anti-PRPF4 antibody [EPR17206(B)] (ab201684) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling PRPF4 with ab201684 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PRPF4 with ab201684 at 1/1200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201684 at 1/1200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Western blot - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Anti-PRPF4 antibody [EPR17206(B)] (ab201684) at 1/5000 dilution + Human fetal spleen lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PRPF4 with ab201684 at 1/1200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201684 at 1/1200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Flow Cytometry - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Flow Cytometry - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

    Flow cytometry analysis of HeLa cells labelling PRPF4 (red) with purified ab201684 at dilution of 1/50. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody. 

  • Immunoprecipitation - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Immunoprecipitation - Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

    PRPF4 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201684 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab201684 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201684 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201684 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-PRPF4 antibody [EPR17206(B)] (ab201684)
    Anti-PRPF4 antibody [EPR17206(B)] (ab201684)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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