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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-CHAMP antibody (ab272554)

Anti-CHAMP antibody (ab272554)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CHAMP
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CHAMP antibody
    See all CHAMP primary antibodies
  • Description

    Rabbit polyclonal to CHAMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CHAMP aa 50-200.
    Database link: Q96JM3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human testis, kidney, colon and lymph node tissue. IF: U-2 OS whole cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)

    Paraffin-embedded formalin-fixed human colon tissue stained for CHAMP using ab272554 at 1/20 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (ab272554)
    Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (ab272554)

    PFA fixed, Triton X-100 permeablized U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate labeling CHAMP (green) using ab272554 at 2 ug/ml in ICC/IF analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)

    Paraffin-embedded formalin-fixed human testis tissue stained for CHAMP using ab272554 at 1/20 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)

    Paraffin-embedded formalin-fixed human kidney tissue stained for CHAMP using ab272554 at 1/20 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAMP antibody (ab272554)

    Paraffin-embedded formalin-fixed human lymph tissue stained for CHAMP using ab272554 at 1/20 dilution in immunohistochemical analysis.

  • Western blot - Anti-CHAMP antibody (ab272554)
    Western blot - Anti-CHAMP antibody (ab272554)
    All lanes : Anti-CHAMP antibody (ab272554) at 0.4 µg/ml

    Lane 1 : siRNA probe #1 transfected into U2OS (human bone osteosarcoma epithelial cell line) cells
    Lane 2 : siRNA probe #2 transfected into U2OS cells
    Lane 3 : siRNA control transfected into U2OS cells

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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