Anti-Estrogen Receptor beta antibody (ab3576)
Key features and details
- Rabbit polyclonal to Estrogen Receptor beta
- Suitable for: ICC, ICC/IF, WB
- Reacts with: Human, Chinese hamster
- Isotype: IgG
Overview
-
Product name
Anti-Estrogen Receptor beta antibody
See all Estrogen Receptor beta primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor beta -
Host species
Rabbit -
Specificity
By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining. -
Tested applications
Suitable for: ICC, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Chinese hamster
Predicted to work with: Hamster
-
Immunogen
Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
Sequence:CSSTEDSKNKESSQNLQSQ
(Peptide available asab5018)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Estrogen Receptor beta antibody (ab3576)All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 nuclear extract
Lane 3 : T47D whole cell lysates
Lane 4 : T47D nuclear extract
Lane 5 : SK-BR-3 whole cell lysates
Lane 6 : SK-BR-3 nuclear extract
Lane 7 : Hs 578T whole cell lysates
Lane 8 : Hs 578T nuclear extract
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Predicted band size: 59 kDaHDAC1 polyclonal antibody (bottom panel) at a dilution of 1/1000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/40,000. Chemiluminescent detection.
-
Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification. -
Western blot - Anti-Estrogen Receptor beta antibody (ab3576)All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution
Lane 1 : CAL region of Human brain - Whole cell lysate
Lane 2 : EC region of Human brain - Whole cell lysate
Lane 3 : HIP region of Human brain - Whole cell lysate
Lane 4 : IT region of Human brain - Whole cell lysate
Lane 5 : MF region of Human brain - Whole cell lysate
Lane 6 : SP region of Human brain - Whole cell lysate
Secondary
All lanes : HRP conjugated Donkey Anti-rabbit IgG
Predicted band size: 59 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This image is courtesy of an Abreview submitted on 3 February 2006. -
Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).
