Anti-CGRP antibody (ab47027)
Key features and details
- Rabbit polyclonal to CGRP
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CGRP antibody
See all CGRP primary antibodies -
Description
Rabbit polyclonal to CGRP -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody has 78% homology with the human CGRP protein. Previous batches of ab47027 have shown to work in human tissue. Please contact Abcam Scientific Support for more information. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Rat CGRP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab47101) -
Positive control
- IHC-P: FFPE rat dorsal root ganglion, spinal cord and rat thyroid tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 0.1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Relevance
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
Cellular localization
Endoplasmic reticulum and Secreted -
Database links
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- SwissProt: P70160 Mouse
- SwissProt: Q99JA0 Mouse
- SwissProt: P01256 Rat
- SwissProt: P01257 Rat
see all -
Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in a Rat normal thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CGRP staining on Rat spinal cord sections using ab47027 (1:400). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
Protocols
References (18)
ab47027 has been referenced in 18 publications.
- Yan D et al. Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis. Sci Rep 9:2698 (2019). PubMed: 30804432
- Wu X et al. SIRT6 abrogation promotes adrenocortical carcinoma through activation of NF-?B signaling. Mol Cell Biochem N/A:N/A (2019). PubMed: 30989475
- de Freitas Rodrigues A et al. The effectiveness of photobiomodulation in the management of temporomandibular pain sensitivity in rats: behavioral and neurochemical effects. Lasers Med Sci N/A:N/A (2019). PubMed: 31292820
- Liao CC et al. Auricular Electrical Stimulation Alleviates Headache through CGRP/COX-2/TRPV1/TRPA1 Signaling Pathways in a Nitroglycerin-Induced Migraine Rat Model. Evid Based Complement Alternat Med 2019:2413919 (2019). PubMed: 31885641
- Xu W et al. Electroacupuncture Relieves Visceral Hypersensitivity by Inactivating Protease-Activated Receptor 2 in a Rat Model of Postinfectious Irritable Bowel Syndrome. Evid Based Complement Alternat Med 2018:7048584 (2018). PubMed: 30420896
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in a Rat normal thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CGRP staining on Rat spinal cord sections using ab47027 (1:400). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
