Anti-CGRP antibody (ab92609)
Key features and details
- Chicken polyclonal to CGRP
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgY
Overview
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Product name
Anti-CGRP antibody
See all CGRP primary antibodies -
Description
Chicken polyclonal to CGRP -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Rat
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Immunogen
Synthetic peptide:
KDNFVPTNVGSEAF-NH2
, corresponding to amino acids 106-119 of Rat CGRP (SwissProt ID: P01256) -
Positive control
- Trigeminal ganglion neurons
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Protein L purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab92609)Immunohistochemical analysis of expression of CGRP using ab92609.
A: the expression of CGRP is shown in the entire trigeminal ganglion obtained from an untreated adult rat.
B: at higher magnification (bar = 20µm), neuronal processes (arrows) expressing CGRP that extend from neuronal cell bodies in one cluster to another cluster are observed.
Protocol: ab92609 was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature.Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with 1/100 ab92609. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were
