Anti-CEP76 antibody (ab86613)
Key features and details
- Rabbit polyclonal to CEP76
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP76 antibody -
Description
Rabbit polyclonal to CEP76 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CEP76 antibody (ab86613)All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 76 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 305 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 3 minutes -
Immunoprecipitation - Anti-CEP76 antibody (ab86613)Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds
All lanes : Anti-CEP76 antibody (ab86613) at 1 µg/ml
Lane 1 : immunoprecipitated CEP76
Lane 2 : control IgG