Anti-Centrin 1 antibody (ab156858)
Key features and details
- Rabbit polyclonal to Centrin 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-Centrin 1 antibody
See all Centrin 1 primary antibodies -
Description
Rabbit polyclonal to Centrin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Xenopus laevis
Predicted to work with: Cow
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Immunogen
Synthetic peptide conjugated to KLH, with a Cysteine residue linker corresponding to C terminal amino acids 155-168 of Human Centrin 1 (Uniprot ID: Q12798).
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Positive control
- HeLa and MCF7 crude cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (ab156858)
ab156858 staining Centrin 1 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000. DAPI (Blue) was used as a nuclear counterstain.
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Western blot - Anti-Centrin 1 antibody (ab156858)All lanes : Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 20 µg
Lane 2 : MCF7 cell lysate at 20 µg
Lane 3 : NIH3T3 cell lysate at 20 µg
Lane 4 : Xenopus eggs cell lysate at 50 µg
Predicted band size: 20 kDa
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Western blot - Anti-Centrin 1 antibody (ab156858)All lanes : Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution
Lane 1 : HeLa crude cell lysate at 10 µg
Lane 2 : MCF7 crude cell lysate at 20 µg
Predicted band size: 20 kDa
