Anti-CENPQ antibody (ab105742)
Key features and details
- Rabbit polyclonal to CENPQ
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPQ antibody -
Description
Rabbit polyclonal to CENPQ -
Host species
Rabbit -
Specificity
Cross-reactivity with CENPQ from other sources has not been determined. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length Human CENPQ (NP_060602.2).
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Positive control
- HeLa whole cell lysate and HeLa cells transfected with control sh-virus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CENPQ antibody (ab105742)All lanes : Anti-CENPQ antibody (ab105742) at 0.5 µg/ml
Lane 1 : Lysates from HeLa cells transfected with control sh-virus
Lane 2 : Lysates from HeLa cells transfected with CENPQ sh-virus
Predicted band size: 31 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CENPQ antibody (ab105742)Immunofluorescence microscopy using ab105742 shows detection of endogenous CENPQ in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENPQ signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENPQ and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide.
