Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)
![Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)](https://www.abcam.com/ps/products/250/ab250547/Images/ab250547-402988-anti-ceecam1-antibody-epr13744-bsa-and-azide-free-western-blot.jpg "Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13744] to CEECAM1 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free
See all CEECAM1 primary antibodies -
Description
Rabbit monoclonal [EPR13744] to CEECAM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250547 is the carrier-free version of ab181979. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250547 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13744 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)All lanes : Anti-CEECAM1 antibody [EPR13744] (ab181979) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaThis data was developed using ab181979, the same antibody clone in a different buffer formulation.
-
![Flow Cytometry - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)](https://www.abcam.com/ps/products/250/ab250547/Images/ab250547-1-anti-ceecam1-antibody-epr13744-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)This data was developed using ab181979, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling CEECAM1 with ab181979 at 1/300 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)](https://www.abcam.com/ps/products/250/ab250547/Images/ab250547-3-benefits-of-recombinant-antibodies.png)
Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (ab250547)
