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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-CDYL antibody (ab5188)

Price and availability

288 134 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-CDYL antibody (ab5188)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CDYL
  • Suitable for: WB
  • Reacts with: Human, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-CDYL antibody
    See all CDYL primary antibodies
  • Description

    Rabbit polyclonal to CDYL
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    African green monkey
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse CDYL aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13740)

  • Positive control

    • This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
African green monkey
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).
Notes
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).

Target

  • Function

    Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
  • Tissue specificity

    Ubiquitous. Expressed at moderate levels in all tissues examined.
  • Sequence similarities

    Contains 1 chromo domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9Y232 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9425 Human
    • Entrez Gene: 12593 Mouse
    • Entrez Gene: 361237 Rat
    • Omim: 603778 Human
    • SwissProt: Q9Y232 Human
    • SwissProt: Q9WTK2 Mouse
    • SwissProt: Q6AYK9 Rat
    • Unigene: 269092 Human
    • Unigene: 29002 Mouse
    • Unigene: 146844 Rat
    see all
  • Alternative names

    • CDY-like antibody
    • CDY-like autosomal antibody
    • Cdyl antibody
    • CDYL: chromodomain protein Y-like antibody
    • CDYL1 antibody
    • CDYL1_HUMAN antibody
    • chromodomain protein Y chromosome-like 6 antibody
    • Chromodomain protein Y like antibody
    • Chromodomain Y-like protein antibody
    • DKFZP586C1622 antibody
    • MGC131936 antibody
    • OTTHUMP00000015984 antibody
    • OTTHUMP00000211185 antibody
    • OTTHUMP00000211186 antibody
    • OTTHUMP00000211189 antibody
    • testis-specific chromodomain Y-like protein antibody
    • Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
    see all

Images

  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)
    All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
    Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
    All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

    Predicted band size: 66 kDa
    Observed band size: 83 kDa why is the actual band size different from the predicted?



    Rabbit polyclonal to CDYL (ab5188) at 1/1000.

    Left lane (-) COS7 (monkey) cells.
    Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (10)

    Publishing research using ab5188? Please let us know so that we can cite the reference in this datasheet.

    ab5188 has been referenced in 10 publications.

    • Qiu Z  et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
    • Wang J  et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
    • Qi C  et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . PubMed: 24671995
    • Escamilla-Del-Arenal M  et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . PubMed: 24144980
    • Bantscheff M  et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol : (2011). PubMed: 21258344
    • Sass S  et al. MicroRNAs coordinately regulate protein complexes. BMC Syst Biol 5:136 (2011). WB ; Human . PubMed: 21867514
    • Zhang Y  et al. Corepressor Protein CDYL Functions as a Molecular Bridge between Polycomb Repressor Complex 2 and Repressive Chromatin Mark Trimethylated Histone Lysine 27. J Biol Chem 286:42414-25 (2011). WB, ChIP, ChIP-sequencing ; Human . PubMed: 22009739
    • Vermeulen M  et al. Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic Histone Marks and Their Readers. Cell 142:967-80 (2010). WB ; Human . PubMed: 20850016
    • Franz H  et al. Multimerization and H3K9me3 Binding Are Required for CDYL1b Heterochromatin Association. J Biol Chem 284:35049-59 (2009). WB ; Human . PubMed: 19808672
    • Kuppuswamy M  et al. Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex. Mol Cell Biol 28:269-81 (2008). WB . PubMed: 17967884

    Images

    • Western blot - Anti-CDYL antibody (ab5188)
      Western blot - Anti-CDYL antibody (ab5188)
      All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml

      Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
      Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 66 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes
    • Western blot - Anti-CDYL antibody (ab5188)
      Western blot - Anti-CDYL antibody (ab5188) Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
      All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution

      Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
      Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

      Predicted band size: 66 kDa
      Observed band size: 83 kDa why is the actual band size different from the predicted?



      Rabbit polyclonal to CDYL (ab5188) at 1/1000.

      Left lane (-) COS7 (monkey) cells.
      Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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