Anti-CDYL antibody (ab5188)
Key features and details
- Rabbit polyclonal to CDYL
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CDYL antibody
See all CDYL primary antibodies -
Description
Rabbit polyclonal to CDYL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey -
Immunogen
Synthetic peptide corresponding to Mouse CDYL aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13740) -
Positive control
- This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB African green monkeyAll applications MouseRatApplication Abreviews Notes WB (1) 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).Notes WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).Target
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Function
Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4. -
Tissue specificity
Ubiquitous. Expressed at moderate levels in all tissues examined. -
Sequence similarities
Contains 1 chromo domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9425 Human
- Entrez Gene: 12593 Mouse
- Entrez Gene: 361237 Rat
- Omim: 603778 Human
- SwissProt: Q9Y232 Human
- SwissProt: Q9WTK2 Mouse
- SwissProt: Q6AYK9 Rat
- Unigene: 269092 Human
see all -
Alternative names
- CDY-like antibody
- CDY-like autosomal antibody
- Cdyl antibody
see all
Images
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All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.
Protocols
References (10)
ab5188 has been referenced in 10 publications.
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Qi C et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . PubMed: 24671995
- Escamilla-Del-Arenal M et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . PubMed: 24144980
- Bantscheff M et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol : (2011). PubMed: 21258344
- Sass S et al. MicroRNAs coordinately regulate protein complexes. BMC Syst Biol 5:136 (2011). WB ; Human . PubMed: 21867514
- Zhang Y et al. Corepressor Protein CDYL Functions as a Molecular Bridge between Polycomb Repressor Complex 2 and Repressive Chromatin Mark Trimethylated Histone Lysine 27. J Biol Chem 286:42414-25 (2011). WB, ChIP, ChIP-sequencing ; Human . PubMed: 22009739
- Vermeulen M et al. Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic Histone Marks and Their Readers. Cell 142:967-80 (2010). WB ; Human . PubMed: 20850016
- Franz H et al. Multimerization and H3K9me3 Binding Are Required for CDYL1b Heterochromatin Association. J Biol Chem 284:35049-59 (2009). WB ; Human . PubMed: 19808672
- Kuppuswamy M et al. Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex. Mol Cell Biol 28:269-81 (2008). WB . PubMed: 17967884
Images
-
All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
-
All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.