Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP54] to CDX2 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CDX2 antibody [SP54] - BSA and Azide free
    See all CDX2 primary antibodies

  • Description

    Rabbit monoclonal [SP54] to CDX2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon carcinoma tissue. Flow Cyt: HT-29 cells. ICC/IF: Caco-2 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab240936 is the carrier-free version of ab101532. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240936 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CDX2 with ab101532 at 1/100 dilution (1.11 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101532 for 30 mins at room temperature.

    This image was generated using ab101532, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Immunocytochemistry/ Immunofluorescence analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with purified ab101532 at 1/50 (2.22 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated using ab101532, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Flow Cytometry - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Flow cytometry analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) labeling CDX2 with purified ab101532 at 1/200 dilution (0.56 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

    This image was generated using ab101532, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Staining of CDX2 in a Formalin/PFA-fixed paraffin-embedded section of human colon carcinoma tissue using ab101532 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101532)

  • Flow Cytometry - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Flow Cytometry - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

    Flow cytometric analysis of rabbit anti-CDX2 (SP54) antibody ab101532(1/100) in HT29 cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101532)

  • Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
    Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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