Anti-CDNF antibody [6G5] (ab205551)
![Anti-CDNF antibody [6G5] (ab205551)](https://www.abcam.com/ps/products/205/ab205551/Images/ab205551-248538-anti-cdnf-antibody-6g5-immunohistochemistry.jpg "Anti-CDNF antibody [6G5] (ab205551)")
Key features and details
- Mouse monoclonal [6G5] to CDNF
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-CDNF antibody [6G5]
See all CDNF primary antibodies -
Description
Mouse monoclonal [6G5] to CDNF -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Chinese hamster
Does not react with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human CDNF.
Database link: Q49AH0 -
Positive control
- WB: CDNF protein expressed into the supernatant of CHO cell culture medium. IHC-P: Human cerebral cortex tissue from Alzheimer’s disease patients. ICC/IF: CDNF expressing U2OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
6G5 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [6G5] (ab205551)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer’s disease patients labeling CDNF with ab205551 at 5 µg/ml. Image on the right shows negative staining without primary antibody. Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium.
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![Western blot - Anti-CDNF antibody [6G5] (ab205551)](https://www.abcam.com/ps/products/205/ab205551/Images/ab205551-248537-anti-cdnf-antibody-6g5-western-blot.jpg)
Western blot - Anti-CDNF antibody [6G5] (ab205551)Anti-CDNF antibody [6G5] (ab205551) at 0.5 µg/ml + CDNF protein expressed into the supernatant of CHO cell culture medium
Predicted band size: 21 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CDNF antibody [6G5] (ab205551)](https://www.abcam.com/ps/products/205/ab205551/Images/ab205551-248539-anti-cdnf-antibody-6g5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDNF antibody [6G5] (ab205551)Immunofluorescent analysis of U2OS cells labeling CDNF with ab205551 at 1 µg/ml. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody. Counterstained with DAPI (blue).
Left panel: CDNF expressing U2OS cells.
Right panel: Negative control (non-transfected U2OS cells).