Anti-CDKN2A/p16INK4a antibody (ab264192)
Key features and details
- Rabbit polyclonal to CDKN2A/p16INK4a
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody
See all CDKN2A/p16INK4a primary antibodies -
Description
Rabbit polyclonal to CDKN2A/p16INK4a -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CDKN2A/p16INK4a aa 106-156. The exact sequence is proprietary.
Database link: P42771 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDKN2A/p16INK4a antibody (ab264192)All lanes : Anti-CDKN2A/p16INK4a antibody (ab264192) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate4-12% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody (ab264192)CDKN2A/p16INK4a was immunoprecipitated from 1mg HeLa cell lysate using ab264192 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab264192 at 1 µg/ml.
Lane 1: ab264192 lot 1 IP in HeLa cell lysate.
Lane 2: ab264192 lot 2 IP in HeLa cell lysate.
Lane 3: Control IgG.Detection: Chemiluminescence with an exposure time of 3 minutes.
Cells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody (ab264192)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CDKN2A/p16INK4a using ab264192 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
