Anti-CD99 antibody [M030] - Extracellular domain (ab253292)
![Anti-CD99 antibody [M030] - Extracellular domain (ab253292)](https://www.abcam.com/ps/products/253/ab253292/Images/ab253292-343905-AntiCD99-antibody-M030--Extracellular-domain-immunocytochemistry-MeWo-human.jpg "Anti-CD99 antibody [M030] - Extracellular domain (ab253292)")
Key features and details
- Mouse monoclonal [M030] to CD99 - Extracellular domain
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD99 antibody [M030] - Extracellular domain
See all CD99 primary antibodies -
Description
Mouse monoclonal [M030] to CD99 - Extracellular domain -
Host species
Mouse -
Specificity
Detects the short and long forms of CD99.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type within Human CD99. The exact sequence is proprietary. Clone M030 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.
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Positive control
- WB: A431, LNCaP, MDA-MB-231, A549 and MeWo cell lysates. ICC/IF: MeWo cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M030 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [M030] - Extracellular domain (ab253292)
Paraformaldehyde-fixed MeWo (human malignant melanoma cell line) cells labeling CD99 using ab253292 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD99 antibody [M030] - Extracellular domain (ab253292)](https://www.abcam.com/ps/products/253/ab253292/Images/ab253292-343904-AntiCD99-antibody-M030--Extracellular-domain-western-blot-A431-LNCaP-MDAMB231-A549-MeWo-human.jpg)
Western blot - Anti-CD99 antibody [M030] - Extracellular domain (ab253292)All lanes : Anti-CD99 antibody [M030] - Extracellular domain (ab253292) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 3 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 4 : MDA-MB-231 (Human cell line) cell lysate
Lane 5 : MeWo (Human malignant melanoma cell line) cell lysate
Predicted band size: 19 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.