Anti-CD99 antibody [M026] - Extracellular domain (ab253291)
![Anti-CD99 antibody [M026] - Extracellular domain (ab253291)](https://www.abcam.com/ps/products/253/ab253291/Images/ab253291-343902-AntiCD99-antibody-M026--Extracellular-domain-immunocytochemistry-MeWo-human.jpg "Anti-CD99 antibody [M026] - Extracellular domain (ab253291)")
Key features and details
- Mouse monoclonal [M026] to CD99 - Extracellular domain
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-CD99 antibody [M026] - Extracellular domain
See all CD99 primary antibodies -
Description
Mouse monoclonal [M026] to CD99 - Extracellular domain -
Host species
Mouse -
Specificity
Detects the short and long forms of CD99.
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Tested applications
Suitable for: ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Other Immunogen Type within Human CD99. The exact sequence is proprietary. Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.
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Positive control
- WB: A431, LNCaP, MDA-MB-231, A549 and MeWo cell lysates. ICC/IF: MeWo cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M026 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [M026] - Extracellular domain (ab253291)
Paraformaldehyde-fixed MeWo (human malignant melanoma cell line) cells labeling CD99 using ab253291 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD99 antibody [M026] - Extracellular domain (ab253291)](https://www.abcam.com/ps/products/253/ab253291/Images/ab253291-343903-AntiCD99-antibody-M026--Extracellular-domain-western-blot-A431-LNCa-MDAMB231-A549-MeWo-human.jpg)
Western blot - Anti-CD99 antibody [M026] - Extracellular domain (ab253291)All lanes : Anti-CD99 antibody [M026] - Extracellular domain (ab253291) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 3 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 4 : MDA-MB-231 (Human cell line) cell lysate
Lane 5 : MeWo (Human malignant melanoma cell line) cell lysate
Predicted band size: 19 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.
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![ELISA - Anti-CD99 antibody [M026] - Extracellular domain (ab253291)](https://www.abcam.com/ps/products/253/ab253291/Images/ab253291-382415-anti-cd99-antibody-m026-extracellular-domain-representative-ELISA-standard-curve.jpg)
ELISA - Anti-CD99 antibody [M026] - Extracellular domain (ab253291)Representative Standard Curve using mouse monoclonal anti-CD99 (ab253291) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
