Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3096] to CD99
Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
Reacts with: Human

Product name

Anti-CD99 antibody [EPR3096]
See all CD99 primary antibodies
Description

Rabbit monoclonal [EPR3096] to CD99
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to the extracellular domains in Human CD99.
Positive control
- THP-1, Jurkat, U937, Molt-4, HuT-78, and HUVEC cell lysates, Human pancreas, Human tonsil and Human uterus tissues
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3096
Isotype

IgG
Research areas

Western blot - Anti-CD99 antibody [EPR3096] (ab108297)

All lanes : Anti-CD99 antibody [EPR3096] (ab108297) at 1/1000 dilution

Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : HuT-78 cell lysate
Lane 6 : HUVEC cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 19 kDa
Observed band size: 28,32 kDa
why is the actual band size different from the predicted?
Immunocytochemistry - Anti-CD99 antibody [EPR3096] (ab108297)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) labelling CD99 with purified ab108297 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] (ab108297)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab108297 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CD99 antibody [EPR3096] (ab108297)

Flow cytometric analysis of Molt-4 cells using anti-CD99 RabMAb (red) (ab108297) or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] (ab108297)

Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using ab108297. Green-CD99 red-PI

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] (ab108297)

Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108297 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] (ab108297)

Immunohistochemical staining of paraffin-embedded Human uterus tissue using ab108297 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD99 antibody [EPR3096] (ab108297)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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