Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR029F] to CD86 - BSA free
  • Suitable for: WB, ICC, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD86 antibody [BLR029F] - BSA free
    See all CD86 primary antibodies

  • Description

    Rabbit monoclonal [BLR029F] to CD86 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD86 (extracellular).
    Database link: P42081

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Images

  • Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    CD86 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243886 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243886 at 1/1000 dilution.

    Lane 1: ab243886 IP in HDLM-2 whole cell lysate.

    Lane 2: Control IgG IP in HDLM-1 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • Western blot - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    Western blot - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

    Western blot analysis using ab243886 at 1/1000 dilution.

    Lane 1: HDLM-2 whole cell lysate (5 µg).

    Lane 2: MJ whole cell lysate (5 µg).

    Lane3: SUP-M2 whole cell lysate (50 µg).

    Lane 4: KG-1 whole cell lysate (50 µg).

    Lane 5: MOLT-4 whole cell lysate (50 µg).

    Lane 6: Ramos whole cell lysate (50 µg).

    Lane 7: Jurkat whole cell lysate (50 µg).

    Lane 8: HeLa whole cell lysate (50 µg).

    Lane 9: HEK-293T whole cell lysate (50 µg).

    Lane 10: RAW 264.7 whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.

  • Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    Formalin-fixed, paraffin-embedded HDLM-2 cells labeling CD86 using ab243886 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
  • Anti-CD86 antibody [BLR029F] - BSA free (ab243886)
    Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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