Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR029F] to CD86 - BSA free
Suitable for: WB, ICC, IP
Reacts with: Mouse, Human

Product name

Anti-CD86 antibody [BLR029F] - BSA free
See all CD86 primary antibodies
Description

Rabbit monoclonal [BLR029F] to CD86 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD86 (extracellular).
Database link: P42081
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR029F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

CD86 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243886 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243886 at 1/1000 dilution.
Lane 1: ab243886 IP in HDLM-2 whole cell lysate.

Lane 2: Control IgG IP in HDLM-1 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Western blot analysis using ab243886 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (5 µg).
Lane 2: MJ whole cell lysate (5 µg).
Lane3: SUP-M2 whole cell lysate (50 µg).
Lane 4: KG-1 whole cell lysate (50 µg).
Lane 5: MOLT-4 whole cell lysate (50 µg).
Lane 6: Ramos whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
Lane 8: HeLa whole cell lysate (50 µg).
Lane 9: HEK-293T whole cell lysate (50 µg).
Lane 10: RAW 264.7 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Formalin-fixed, paraffin-embedded HDLM-2 cells labeling CD86 using ab243886 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

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