Anti-CD73 antibody (ab175396)
Key features and details
- Rabbit polyclonal to CD73
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CD73 antibody
See all CD73 primary antibodies -
Description
Rabbit polyclonal to CD73 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human CD73.
Database link: P21589 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody (ab175396)
Paraffin-embedded human placenta stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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Western blot - Anti-CD73 antibody (ab175396)All lanes : Anti-CD73 antibody (ab175396) at 1/1000 dilution
Lane 1 : DU145 cell extract
Lane 2 : Mouse liver extract
Lane 3 : Mouse lung extract
Lane 4 : Rat brain extract
Lane 5 : Rat liver extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody (ab175396)Paraffin-embedded rat spleen stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody (ab175396)Paraffin-embedded human placenta stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody (ab175396)Paraffin-embedded human liver tissue stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
