Anti-CD73 antibody [AD2] (ab130451)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AD2] to CD73
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD73 antibody [AD2]
See all CD73 primary antibodies -
Description
Mouse monoclonal [AD2] to CD73 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human PBMCs.
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General notes
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AD2 -
Isotype
IgG1 -
Research areas
Images
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Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).
Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.
Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.
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