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Anti-CD73 antibody [AD2] (ab130451)

Price and availability

307 566 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-CD73 antibody [AD2] (ab130451)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AD2] to CD73
  • Suitable for: Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD73 antibody [AD2]
    See all CD73 primary antibodies
  • Description

    Mouse monoclonal [AD2] to CD73
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: Human PBMCs.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 21st September 2020.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    AD2
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Images

  • Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)
    Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)

    Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).

    Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.

    Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.

  • Anti-CD73 antibody [AD2] (ab130451)
    Anti-CD73 antibody [AD2] (ab130451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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