Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22065] to CD7 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD7 antibody [EPR22065] - BSA and Azide free
    See all CD7 primary antibodies

  • Description

    Rabbit monoclonal [EPR22065] to CD7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MOLT-4 cells.

  • General notes

    Ab239755 is the carrier-free version of ab224194. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239755 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22065

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)
    Flow Cytometry - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)

    Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID: 3260936)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

  • Immunoprecipitation - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)
    Immunoprecipitation - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)

    CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 μg (Input).
    Lane 2: ab224194 IP in human tonsil lysate lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)
    Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PDS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID: 16332967).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

  • Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)
    Anti-CD7 antibody [EPR22065] - BSA and Azide free (ab239755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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