Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20544] to CD3D - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD3D antibody [EPR20544] - BSA and Azide free
    See all CD3D primary antibodies

  • Description

    Rabbit monoclonal [EPR20544] to CD3D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IP, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus and lymph node lysates. IHC-P: Mouse spleen and thymus tissues. IHC-Fr: Mouse spleen tissue. Flow Cyt: Mouse spleen cells. IP: Mouse thymus lysate.

  • General notes

    Ab229280 is the carrier-free version of ab213362. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229280 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20544

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)

    This data was developed using ab213362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on T cells of mouse splenic periarterial lymphatic sheath is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Frozen sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Immunohistochemistry (Frozen sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    This data was developed using ab213362, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD3D with ab213362 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and membranous staining on mouse splenic periarterial lymphatic sheath is observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Western blot - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Western blot - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    All lanes : Anti-CD3D antibody [EPR20544] (ab213362) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Mouse lymph node tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab213362, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lane 2: 3 minutes.

  • Flow Cytometry - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Flow Cytometry - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    This data was developed using ab213362, the same antibody clone in a different buffer formulation.Flow cytometric analysis of mouse spleen cells labeling CD3D with ab213362 at 1/600 dilution (right panel), compared with a rabbit monoclonal IgG isotype control (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Firstly, cells surface was stained with anti-mouse CD3 Alexa Fluor 647 (Y axis), then cells were fixed with 4% PFA followed by intracellular staining with ab213362. The same population of cells were stained by both: anti-mouse CD3 and anti-mouse CD3D (ab213362) antibodies.
  • Immunoprecipitation - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Immunoprecipitation - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)

    This data was developed using ab213362, the same antibody clone in a different buffer formulation.

    CD3D was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab213362 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213362 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse thymus lysate, 10 µg (Input).

    Lane 2: ab213362 IP in mouse thymus lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213362 in mouse thymus lysate .

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    This data was developed using ab213362, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining on T cells of mouse thymus medulla is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)
    Anti-CD3D antibody [EPR20544] - BSA and Azide free (ab229280)

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