Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD35 antibody [EPR6601] (ab133293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6601] to CD35
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD35 antibody [EPR6601]
    See all CD35 primary antibodies

  • Description

    Rabbit monoclonal [EPR6601] to CD35

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of human CD35. (P17927).

  • Positive control

    • WB: TF-1, and Human tonsil lysates. IHC-P: human tonsil, kidney tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD35 with purified ab133293 at 1:250 dilution (1.76 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CD35 antibody [EPR6601] (ab133293)
    Western blot - Anti-CD35 antibody [EPR6601] (ab133293)
    Anti-CD35 antibody [EPR6601] (ab133293) at 1/10000 dilution (Purified) + Human tonsil lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 223 kDa
    Observed band size: 280 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-CD35 antibody [EPR6601] (ab133293)
    Western blot - Anti-CD35 antibody [EPR6601] (ab133293)
    Anti-CD35 antibody [EPR6601] (ab133293) at 1/1000 dilution (Purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 223 kDa
    Observed band size: 280 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling CD35 with unpurified ab133293 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD35 with 1/100 unpurified ab133293.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD35 antibody [EPR6601] (ab133293)
    OI-RD Scanning - Anti-CD35 antibody [EPR6601] (ab133293)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD35 antibody [EPR6601] (ab133293)
    Anti-CD35 antibody [EPR6601] (ab133293)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD35 antibody [EPR6601] (ab133293)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.