Anti-CD35 antibody [EPR6601] (ab133293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6601] to CD35
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-CD35 antibody [EPR6601]
See all CD35 primary antibodies -
Description
Rabbit monoclonal [EPR6601] to CD35 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
A synthetic peptide corresponding to residues in the extracellular domain of human CD35. (P17927).
-
Positive control
- WB: TF-1, and Human tonsil lysates. IHC-P: human tonsil, kidney tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Dissociation constant (KD)
KD = 5.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6601 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD35 with purified ab133293 at 1:250 dilution (1.76 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Anti-CD35 antibody [EPR6601] (ab133293) at 1/10000 dilution (Purified) + Human tonsil lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
-
Anti-CD35 antibody [EPR6601] (ab133293) at 1/1000 dilution (Purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling CD35 with unpurified ab133293 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (ab133293)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD35 with 1/100 unpurified ab133293.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-