Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP197] to CD35 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-CD35 antibody [SP197] - BSA and Azide free
See all CD35 primary antibodies
Description

Rabbit monoclonal [SP197] to CD35 - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human tonsil lysate. Human kidney, tonsil, colon and spleen tissue.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240961 is the carrier-free version of ab183359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP197
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD35 with ab183359 at 1/100 dilution (1.77 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the glomerular podocytes in the human kidney, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183359 for 10 mins at room temperature.

This image was generated using ab183359, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD35 with ab183359 at 1/100 dilution (1.77 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183359 for 10 mins at room temperature.

This image was generated using ab183359, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue sections labeling CD35 using ab183359 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue sections labeling CD35 using ab183359 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon tissue sections labeling CD35 using ab183359 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue sections labeling CD35 using ab183359 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)
Anti-CD35 antibody [SP197] - BSA and Azide free (ab240961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com