Anti-CD35 antibody [E11] - BSA and Azide free (ab25)
![Anti-CD35 antibody [E11] - BSA and Azide free (ab25)](https://www.abcam.com/ps/products/0/ab25/Images/ab25-3155-anti-cd35-antibody-e11-immunohistochemistry.jpg "Anti-CD35 antibody [E11] - BSA and Azide free (ab25)")
Key features and details
- Mouse monoclonal [E11] to CD35 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD35 antibody [E11] - BSA and Azide free
See all CD35 primary antibodies -
Description
Mouse monoclonal [E11] to CD35 - BSA and Azide free -
Host species
Mouse -
Specificity
Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
Database link: P17927
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E11 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)ab25 (1µg/ml) staining CD35 in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
