Anti-CD34 antibody [My10] (ab245689)
Key features and details
- Rabbit monoclonal [My10] to CD34
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CD34 antibody [My10]
See all CD34 primary antibodies -
Description
Rabbit monoclonal [My10] to CD34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD34. KG-1a Human myeloblastic leukemic cell line (immature human myeloid cell line) from a patient with non-lymphocytic leukemia.
-
Positive control
- IHC-P: Human placenta tissue. WB: Jurkat cell lysate. Flow Cyt: Human leukocytes.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
My10 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
-
Paraffin embedded human placenta tissue stained for CD34 with ab245689 at 4 µg/ml for 30 mins in immunohistochemical analysis. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3′-diaminobenzidine), and counterstaining with hemotoxylin.
-
Human leukocytes were stained with an isotype control (Panel A) or ab245689 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
-
Anti-CD34 antibody [My10] (ab245689) at 0.1 µg/ml (incubated for 1 hr) + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate in RIPA buffer at 35 µg
10% SDS PAGE gel.
Detected by chemiluminescence.