Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2999] to CD34 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD34 antibody [EPR2999] - BSA and Azide free
    See all CD34 primary antibodies

  • Description

    Rabbit monoclonal [EPR2999] to CD34 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247928 is the carrier-free version of ab110643 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247928 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution

    Lane 1 : Human bone marrow tissue lysate
    Lane 2 : TF-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

    ab110643 showing positive staining in Normal stomach vessels tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

    ab110643 showing positive staining in Normal placenta vessels tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

    ab110643 showing positive staining in Normal lung vessels tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

    ab110643 showing positive staining in Normal breast vessels tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using ab110643, the same antibody clone in a different buffer formulation.

    ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    This data was developed using ab110643, the same antibody clone in a different buffer formulation.ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)
    Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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