Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)
![Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)](https://www.abcam.com/ps/products/232/ab232533/Images/ab232533-308923-anti-cd31-antibody-epr17260-263-immunofluorescence.jpg "Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-263] to CD31 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CD31 antibody [EPR17260-263] - BSA and Azide free
See all CD31 primary antibodies -
Description
Rabbit monoclonal [EPR17260-263] to CD31 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: bEND.3 cells.
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General notes
Ab232533 is the carrier-free version of ab222783. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232533 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17260-263 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control: NIH/3T3 (PMID: 1429859).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222783).
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![Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)](https://www.abcam.com/ps/products/232/ab232533/Images/ab232533-2-benefits-of-recombinant-antibodies.png)
Anti-CD31 antibody [EPR17260-263] - BSA and Azide free (ab232533)
