Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)
![Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)](https://www.abcam.com/ps/products/270/ab270034/Images/ab270034-381399-CD28-antibody-EPR2328063-flow-cytometry-mouse-splenocytes.jpg "Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23280-63] to CD28 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD28 antibody [EPR23280-63] - BSA and Azide free
See all CD28 primary antibodies -
Description
Rabbit monoclonal [EPR23280-63] to CD28 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Mouse splenocytes.
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General notes
ab270034 is the carrier-free version of ab246517. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab270034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23280-63 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)
Flow cytometric analysis of mouse splenocytes labelling CD28 with ab246517 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab246517 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246517).
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![Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)](https://www.abcam.com/ps/products/270/ab270034/Images/ab270034-2-benefits-of-recombinant-antibodies.png)
Anti-CD28 antibody [EPR23280-63] - BSA and Azide free (ab270034)
