Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
![Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)](https://www.abcam.com/ps/products/226/ab226152/Images/ab226152-2-anti-cd239-bcam-antibody-epr4165-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4165] to CD239/BCAM - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free
See all CD239/BCAM primary antibodies -
Description
Rabbit monoclonal [EPR4165] to CD239/BCAM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysate, HeLa cell lysate, A431 cell lysate, Human tonsil tissue
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General notes
ab226152 is the carrier-free version of ab134110 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab226152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as CD239
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4165 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)This data was developed using ab134110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD239/BCAM with ab134110 at 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)](https://www.abcam.com/ps/products/226/ab226152/Images/ab226152-399317-anti-cd239-bcam-antibody-epr4165-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)All lanes : Anti-CD239/BCAM antibody [EPR4165] (ab134110) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?This data was developed using ab134110, the same antibody clone in a different buffer formulation.
Secondary antibody - goat anti-rabbit HRP (ab6721).
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![Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)](https://www.abcam.com/ps/products/226/ab226152/Images/ab226152-3-benefits-of-recombinant-antibodies.png)
Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
