Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4165] to CD239/BCAM - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free
    See all CD239/BCAM primary antibodies

  • Description

    Rabbit monoclonal [EPR4165] to CD239/BCAM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T cell lysate, HeLa cell lysate, A431 cell lysate, Human tonsil tissue

  • General notes

    ab226152 is the carrier-free version of ab134110 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab226152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CD239

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
    This data was developed using ab134110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD239/BCAM with ab134110 at 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
    Western blot - Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
    All lanes : Anti-CD239/BCAM antibody [EPR4165] (ab134110) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab134110, the same antibody clone in a different buffer formulation.

    Secondary antibody - goat anti-rabbit HRP (ab6721).

  • Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)
    Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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