Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)
![Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)](https://www.abcam.com/ps/products/22/ab22353/Images/ab22353-2-ab22353Flow1.jpg "Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)")
Key features and details
- Mouse monoclonal [MRC OX-2] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD200 / OX2 antibody [MRC OX-2]
See all CD200 / OX2 primary antibodies -
Description
Mouse monoclonal [MRC OX-2] to CD200 / OX2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD200/ OX2.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Primary antibody notes
This antibody is a useful marker for the follicular dendritic cells in lymphoid tissues as it does not label the dendritic (interdigitating) cells in T areas that are Ia antigen positive. -
Clonality
Monoclonal -
Clone number
MRC OX-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)
Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.