Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14643] to CD163 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD163 antibody [EPR14643] - BSA and Azide free
    See all CD163 primary antibodies

  • Description

    Rabbit monoclonal [EPR14643] to CD163 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250965 is the carrier-free version of ab188571. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250965 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    This data was developed using ab188571, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CD163 with ab188571 at 1/500 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    This data was developed using ab188571, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab188571 at 1/500. A prediluted HRP Polymer for Rabbit IgG was use as the secondary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    Western blot - Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    All lanes : Anti-GIPR antibody - N-terminal (ab188591) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human placenta lysate
    Lane 3 : Human breast cancer lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 125 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab188571, the same antibody clone in a different buffer formulation.

     

  • Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)
    Anti-CD163 antibody [EPR14643] - BSA and Azide free (ab250965)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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