Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)
![Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)](https://www.abcam.com/ps/products/248/ab248421/Images/ab248421-402522-anti-cd11c-antibody-epr1346-bsa-and-azide-free-western-blot.jpg "Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1346] to CD11c - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CD11c antibody [EPR1346] - BSA and Azide free
See all CD11c primary antibodies -
Description
Rabbit monoclonal [EPR1346] to CD11c - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248421 is the carrier-free version of ab133246 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248421 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1346 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)All lanes : Anti-CD11c antibody [EPR1346] (ab133246) at 1/1000 dilution
Lane 1 : HL60 cell lysate, TPA stimulated
Lane 2 : THP1 cell lysate, TPA stimulated
Lysates/proteins at 10 µg per lane.
Predicted band size: 127 kDaThis data was developed using ab133246, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)](https://www.abcam.com/ps/products/248/ab248421/Images/ab248421-399137-anti-cd11c-antibody-epr1346-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)All lanes : Anti-CD11c antibody [EPR1346] (ab133246) at 1/1000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 127 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis data was developed using ab133246, the same antibody clone in a different buffer formulation.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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![Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)](https://www.abcam.com/ps/products/248/ab248421/Images/ab248421-3-benefits-of-recombinant-antibodies.png)
Anti-CD11c antibody [EPR1346] - BSA and Azide free (ab248421)
