Anti-CD11b antibody (ab62817)
Key features and details
- Goat polyclonal to CD11b
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CD11b antibody
See all CD11b primary antibodies -
Description
Goat polyclonal to CD11b -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
-
Immunogen
Synthetic peptide:
C-RNDGEDSYRTQ
, corresponding to internal sequence amino acids 807-817 of Human CD11b -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62817 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody (ab62817)
ab62817 (2µg/ml) staining of paraffin embedded Human Brain shows staining of microglia following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.
-
Western blot - Anti-CD11b antibody (ab62817)Anti-CD11b antibody (ab62817) at 0.3 µg/ml + Human Brain (Cerebellum) lysate (in RIPA buffer) at 35 µg/ml
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody (ab62817)Paraformaldehyde-fixed, 0.15% Triton permeabilized U937 cells stained for CD11B (green) using ab62734 (10ug/ml) in ICC/IF followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Nucelar counterstain: DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
