Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)
![Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)](https://www.abcam.com/ps/products/276/ab276049/Images/ab276049-2-AntiCCR1-antibody-002-IP-HeLa-293T-cells.jpg "Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [002] to CCR1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-CCR1 antibody [002] - BSA and Azide free
See all CCR1 primary antibodies -
Description
Rabbit monoclonal [002] to CCR1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CCR1.
Database link: P32246 -
Positive control
- IP: HeLa and HEK-293T cells. WB: HEK-293 and HEK-293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
002 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CCR1 antibody [002] (ab276049)
CCR1/CD191 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (lane A) and HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (lane B) whole cell lysate with 2 µL ab276049 and 15 µl of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab276049 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibodyat 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions. -
![Western blot - Anti-CCR1 antibody [002] (ab276049)](https://www.abcam.com/ps/products/276/ab276049/Images/ab276049-1-AntiCCR1-antibody-002-wb-HEK293-293T-cells.jpg)
Western blot - Anti-CCR1 antibody [002] (ab276049)All lanes : Anti-CCR1 antibody [002] - BSA and Azide free (ab276049) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 41 kDaDeveloped using the Odyssey technique.
Performed under reducing conditions. -
![Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)](https://www.abcam.com/ps/products/276/ab276049/Images/ab276049-3-benefits-of-recombinant-antibodies.png)
Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)
