Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-cbx7 antibody (ab21873)

Key features and details

  • Rabbit polyclonal to cbx7
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-cbx7 antibody
    See all cbx7 primary antibodies

  • Description

    Rabbit polyclonal to cbx7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee

  • Immunogen

    Synthetic peptide within Human cbx7 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O95931
    (Peptide available as ab22424)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P
Use a concentration of 1 µg/ml.

Target

  • Relevance

    Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • Cbx 7 antibody
      • CBX7 antibody
      • Chromobox homolog 7 antibody
      • Chromobox protein homolog 7 antibody

    Images

    • Western blot - Anti-cbx7 antibody (ab21873)
      Western blot - Anti-cbx7 antibody (ab21873)
      All lanes : Anti-cbx7 antibody (ab21873) at 1 µg

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)
      Lane 3 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 28 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody - ChIP Grade (ab21873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody - ChIP Grade (ab21873)

      IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)
      Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)
      All lanes : Anti-cbx7 antibody (ab21873) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : Jurkat nuclear extract lysate (ab14844)
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 28 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    References (40)

    Publishing research using ab21873? Please let us know so that we can cite the reference in this datasheet.

    ab21873 has been referenced in 40 publications.

    • Tian P  et al. Decreased chromobox homologue 7 expression is associated with epithelial-mesenchymal transition and poor prognosis in cervical cancer. Open Med (Wars) 16:410-418 (2021). PubMed: 33748425
    • Liu Y  et al. EZH2 regulates sFRP4 expression without affecting the methylation of sFRP4 promoter DNA in colorectal cancer cell lines. Exp Ther Med 20:33 (2020). PubMed: 32952624
    • Cho KW  et al. Mammalian CBX7 isoforms p36 and p22 exhibit differential responses to serum, varying functions for proliferation, and distinct subcellular localization. Sci Rep 10:8061 (2020). PubMed: 32415167
    • Hu S  et al. ncHMR detector: a computational framework to systematically reveal non-classical functions of histone modification regulators. Genome Biol 21:48 (2020). PubMed: 32093739
    • Lamb KN  et al. Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7. Cell Chem Biol 26:1365-1379.e22 (2019). PubMed: 31422906
    View all Publications for this product

    Images

    • Western blot - Anti-cbx7 antibody (ab21873)
      Western blot - Anti-cbx7 antibody (ab21873)
      All lanes : Anti-cbx7 antibody (ab21873) at 1 µg

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)
      Lane 3 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 28 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody - ChIP Grade (ab21873)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody - ChIP Grade (ab21873)

      IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)
      Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)
      All lanes : Anti-cbx7 antibody (ab21873) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : Jurkat nuclear extract lysate (ab14844)
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 28 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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