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Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10723(B)] to Cathepsin V - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free
    See all Cathepsin V primary antibodies

  • Description

    Rabbit monoclonal [EPR10723(B)] to Cathepsin V - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249378 is the carrier-free version of ab166894.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    Western blot - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : Fetal kidney tissue lysate
    Lane 5 : Human small intestine tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab166894, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    This data was developed using ab166894, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
  • Immunocytochemistry - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    Immunocytochemistry - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    This data was developed using ab166894, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
  • Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    This data was developed using ab166894, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)
    Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (ab249378)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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