Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free
See all CARM1 primary antibodies
Description

Rabbit monoclonal [EPR23678-113] to CARM1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ChIP,ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HEK-293T; HeLa; HCT116; NIH/3T3 whole cell lysates; Mouse liver and lung tissue lysates; Rat liver tissue lysate. IHC-P: Human colon and colon carcinoma; Mouse liver; Rat colon. Flow Cyt: Wild-type HEK-293T, HeLa cells.
General notes
ab279344 is the carrier-free version of ab243638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab279344 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23678-113
Isotype

IgG
Research areas

Western blot - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : CARM1 knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: Intercept^® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

Lanes 1 - 4: Merged signal (red and green). Green - ab243638 observed at 65 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

ab243638 was shown to react with CARM1 in wild-type HEK-293T cells in western blot with loss of signal observed in CARM1 knockout cell line ab266557 (CARM1 knockout cell lysate ab257871). Wild-type and CARM1 knockout HEK-293T cell lysates were subjected to SDS-PAGE.

ab243638 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

Boiled samples are used in this blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HEK-293T (human embryonic kidney epithelial cell, Right panel)/ CARM1 knockout HEK-293T cells (Left panel) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Positive staining on HEK-293T cells (ab255449), while no staining on CARM1 knockout HEK-293T cells (ab266557).
Western blot - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

This data was developed using ab243638, the same antibody clone in a different buffer formulation

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates

Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon carcinoma (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates

Exposure times:

Lane 1-2: 15 seconds

Lane 3: 92 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)

This data was developed using ab243638, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.