Anti-CARD15/NOD2 antibody (ab36836)
Key features and details
- Rabbit polyclonal to CARD15/NOD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CARD15/NOD2 antibody
See all CARD15/NOD2 primary antibodies -
Description
Rabbit polyclonal to CARD15/NOD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to CARD15/NOD2 (C terminal).
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Positive control
- HeLa cell lysate.
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General notes
This antibody has no cross-reaction with NOD1.
This product was previously labelled as CARD15
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
This antibody has no cross-reaction with NOD1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CARD15/NOD2 antibody (ab36836)Anti-CARD15/NOD2 antibody (ab36836) at 2 µg/ml + HeLa cell lysate
Predicted band size: 115 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-CARD15/NOD2 antibody (ab36836)ab36836 at 10µg/ml staining CARD15/NOD2 in Hela cells by Immunocytochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARD15/NOD2 antibody (ab36836)IHC image of ab36836 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36836, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-CARD15/NOD2 antibody (ab36836)Immunofluorescence of NOD2 in HeLa cells using ab36836 at 20 ug/ml.
