Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit Monoclonal [EPCEAR7] to CEACAM5 - BSA and Azide free
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CEACAM5 antibody [EPCEAR7] - BSA and Azide free
See all CEACAM5 primary antibodies
Description

Rabbit Monoclonal [EPCEAR7] to CEACAM5 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Full length native protein (purified) corresponding to Human Carcino Embryonic Antigen CEA.
Positive control
- Human colon cancer lysate, Human colon carcinoma tissue, MCF-7 and human gastric carcinoma.
General notes
ab226144 is the carrier-free version of ab133633. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab226144 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc

Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPCEAR7
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

Confocal image showing membranous staining in MCF7 cell line.

Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labelling carcino embryonic antigen (CEA) with ab133633 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/10000 dilution was used as the secondary antibody. The cells were counterstained with ab195889, a anti-alpha tubulin antibody at 1/200 dilution. DAPI was used as a nuclear counterstain.

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Carcino Embryonic Antigen CEA with Purified ab133633 at 1:3000 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling Carcino Embryonic Antigen CEA with Purified ab133633 at 1:3000 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing positive staining in Normal colon tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labelling Carcino Embryonic Antigen CEA with ab133633 at 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing positive staining in Stomach adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing negative staining in Skeletal muscle tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing negative staining in Normal tonsil tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing negative staining in Thyroid gland carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing negative staining in Hepatocellular carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

ab133633 showing negative staining in Glioma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133633).
Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] - BSA and Azide free (ab226144)

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