Anti-CaMKII beta antibody (ab34703)
Key features and details
- Rabbit polyclonal to CaMKII beta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-CaMKII beta antibody
See all CaMKII beta primary antibodies -
Description
Rabbit polyclonal to CaMKII beta -
Host species
Rabbit -
Specificity
This antibody does cross-react with CAMKII alpha protein -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse CaMKII beta aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab27843)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Target
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Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Tissue specificity
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 12323 Mouse
- Entrez Gene: 24245 Rat
- SwissProt: P28652 Mouse
- SwissProt: P08413 Rat
- Unigene: 439733 Mouse
- Unigene: 9743 Rat
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Form
The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. -
Alternative names
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase IIB antibody
see all
Images
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Western blot - Anti-CaMKII beta antibody (ab34703)All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml
Lane 1 : Brain Mouse Tissue Lysate
Lane 2 : Brain Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CaMKII beta antibody (ab34703)ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (27)
ab34703 has been referenced in 27 publications.
- van de Looij Y et al. Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups. Front Endocrinol (Lausanne) 10:46 (2019). PubMed: 30800096
- Kool MJ et al. CAMK2-dependent signaling in neurons is essential for survival. J Neurosci N/A:N/A (2019). PubMed: 31064859
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
- Raina A et al. Neuronal Trans-differentiation by Transcription Factors Ascl1 and Nurr1: Induction of a Dopaminergic Neurotransmitter Phenotype in Cortical GABAergic Neurons. Mol Neurobiol N/A:N/A (2019). PubMed: 31317490
- Mahajani S et al. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Dis 10:898 (2019). PubMed: 31776327
Images
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Western blot - Anti-CaMKII beta antibody (ab34703)All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml
Lane 1 : Brain Mouse Tissue Lysate
Lane 2 : Brain Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CaMKII beta antibody (ab34703)ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.